Yijia LI 6 25 2018

Date:6/25/2018

Miniprep of 994

Date:6/27/2018

PCR994

PCR1000_998_129

PCR995

3225subclone

3199subclone

941subclone

Vector digestion_3204cubclone_931-1 subclone

3196subclone

3215subclone

Date:6/28/2018

Vector digestion and 931-1 subclone

Vector digestion and 2509 subclone

Miniprep digestion_3196_3199_3210

Miniprep digestion _3204_3215_941

Date:6/29/2018

Vector digestion 998_1000_931-1_3206_129

998_1000_931-1 use 2015 as vector, Amp

129 vector is 1801

3206 1st PCR

3206 2nd PCR

Miniprep digestion of 3218_995_3197