User contributions for Paul.xu

31 May 2011

• 08:4308:43, 31 May 2011 diff hist +11,425 N Lentivirus Safety 2 Created page with "'''General safety precautions:'''<br>1. All laboratory personals should wear proper personal protective equipment including a laboratory coat, gloves and goggles. These items wou..." current
• 08:4108:41, 31 May 2011 diff hist +3,856 N Lentivirus Safety Created page with "<u>'''Standard Operating Procedures (SOP) for Working Safely with Lentiviral Vectors'''</u> The following personal protective equipment MUST be worn when working with Lentiviral..." current

27 May 2011

• 10:4510:45, 27 May 2011 diff hist +46 N EndoToxin-Free Plasmid-Prep moved EndoToxin-Free Plasmid-Prep to EndoToxin-Free Maxi Plasmid-Prep current
• 09:1209:12, 27 May 2011 diff hist +74 N Lentivirus Purification PEG + Q Membrane + FPLC for in vivo moved Lentivirus Purification PEG + Q Membrane + FPLC for in vivo to Lentivirus Purification PEG + Q Membrane by FPLC for in vivo current
• 09:1209:12, 27 May 2011 diff hist +73 N Lentivirus Purification Q FPLC for in vivo moved Lentivirus Purification Q FPLC for in vivo to Lentivirus Purification PEG + Q Membrane + FPLC for in vivo current
• 09:1109:11, 27 May 2011 diff hist +66 N Lentivirus Purification Q for in vivo moved Lentivirus Purification Q for in vivo to Lentivirus Purification PEG + Q Membrane for in vivo current
• 09:0609:06, 27 May 2011 diff hist +49 N Lentivirus Concentration Q moved Lentivirus Concentration Q to Lentivirus Concentration Q Membrane current

26 May 2011

• 17:2517:25, 26 May 2011 diff hist +510 N SacB selection Created page with "<u>'''sacB selection Protocol'''</u><br> <br> '''For pFastbac-dual or pFastbac vector'''<br> 1. Digest protein kinase in pDNR-Daul vector with Sal1-HindIII.<br> 2. Digest pFa..." current

25 May 2011

• 08:4108:41, 25 May 2011 diff hist +1,152 PKA-alpha-Western-blot No edit summary current
• 07:4407:44, 25 May 2011 diff hist +6,982 Calcium phosphate transfection protocol No edit summary current

22 May 2011

• 19:0119:01, 22 May 2011 diff hist +324 Transfection reagents lots QC No edit summary current
• 18:5918:59, 22 May 2011 diff hist 0 N File:Compared GeneExpresso Max lots 5 22 2011.jpg No edit summary current
• 18:2318:23, 22 May 2011 diff hist +820 Lentivirus-packaging-mix-QC No edit summary current
• 18:2118:21, 22 May 2011 diff hist 0 N File:PVSVG pGagPol QC 5 22 2011.JPG No edit summary current

21 May 2011

• 17:1717:17, 21 May 2011 diff hist 0 N File:Compare GEmax lots.JPG No edit summary current
• 17:1617:16, 21 May 2011 diff hist 0 N File:Compared GeneExpresso Max lots.jpg No edit summary current

20 May 2011

• 16:2216:22, 20 May 2011 diff hist 0 N File:Sigma packaging mix EcoR1.jpg No edit summary current
• 16:2116:21, 20 May 2011 diff hist 0 N File:Sigma packaging mix EcoR1 2.JPG No edit summary current
• 16:2116:21, 20 May 2011 diff hist 0 N File:Sigma packaging EcoR1.JPG No edit summary current
• 16:2116:21, 20 May 2011 diff hist 0 N File:Sigma packaging EcoR1-xho1-Nco1.JPG No edit summary current

6 May 2011

• 19:2619:26, 6 May 2011 diff hist +31 N PKA Alpha pCMV-FC Cloning moved PKA Alpha pCMV-FC Cloning to PKA Alpha pCMV-FC current
• 19:2619:26, 6 May 2011 diff hist +20 m PKA Alpha pCMV-FC moved PKA Alpha pCMV-FC Cloning to PKA Alpha pCMV-FC current
• 19:2619:26, 6 May 2011 diff hist +21 N PEDFR Cloning-2 moved PEDFR Cloning-2 to PEDFR 2 current
• 19:2619:26, 6 May 2011 diff hist +199 m PEDFR 2 moved PEDFR Cloning-2 to PEDFR 2 current
• 19:2519:25, 6 May 2011 diff hist +19 N PEDFR Cloning moved PEDFR Cloning to PEDFR current
• 19:2519:25, 6 May 2011 diff hist +1,709 m PEDFR moved PEDFR Cloning to PEDFR current
• 19:2519:25, 6 May 2011 diff hist +26 N MPEDFR-shRNA-cloning moved MPEDFR-shRNA-cloning to MPEDFR-shRNA current
• 19:2519:25, 6 May 2011 diff hist +289 m MPEDFR-shRNA moved MPEDFR-shRNA-cloning to MPEDFR-shRNA current
• 19:2419:24, 6 May 2011 diff hist +34 N PDB HIS MBP Strep II cloning moved PDB HIS MBP Strep II cloning to PDB HIS MBP Strep II current
• 19:2419:24, 6 May 2011 diff hist +339 m PDB HIS MBP Strep II moved PDB HIS MBP Strep II cloning to PDB HIS MBP Strep II current
• 19:2419:24, 6 May 2011 diff hist +34 N PDB HIS GST Strep II cloning moved PDB HIS GST Strep II cloning to PDB HIS GST Strep II current
• 19:2419:24, 6 May 2011 diff hist +660 m PDB HIS GST Strep II moved PDB HIS GST Strep II cloning to PDB HIS GST Strep II current
• 19:2419:24, 6 May 2011 diff hist +18 N VP16 Cloning moved VP16 Cloning to VP16 current
• 19:2419:24, 6 May 2011 diff hist +3,275 m VP16 moved VP16 Cloning to VP16 current
• 19:2419:24, 6 May 2011 diff hist +22 N TurboTEV cloning moved TurboTEV cloning to TurboTEV current
• 19:2319:23, 6 May 2011 diff hist +42 N PLVXPlus2-IRES-mCherry-PEDFR cloning moved PLVXPlus2-IRES-mCherry-PEDFR cloning to PLVXPlus2-IRES-mCherry-PEDFR current
• 19:2319:23, 6 May 2011 diff hist +345 m PLVXPlus2-IRES-mCherry-PEDFR moved PLVXPlus2-IRES-mCherry-PEDFR cloning to PLVXPlus2-IRES-mCherry-PEDFR current
• 19:1019:10, 6 May 2011 diff hist 0 N File:PDBHisGST.StrepoII digestion Xba1 Pst1.JPG No edit summary current

5 May 2011

• 18:1818:18, 5 May 2011 diff hist 0 N File:PDB HIS MBP Strep II Xba1-Pst1 5 5 2011.JPG No edit summary current
• 18:1618:16, 5 May 2011 diff hist 0 N File:PDB HIS MBP Strep II Xba1-Pst1 5-5-2011.JPG No edit summary current

3 May 2011

• 17:1817:18, 3 May 2011 diff hist +3,292 PKA Alpha pLVX-Plus2 No edit summary current
• 17:1517:15, 3 May 2011 diff hist 0 N File:PLVXPlus2-PKA-alpha 2011-5-3 digestion.JPG No edit summary current
• 17:1017:10, 3 May 2011 diff hist 0 N File:IMG 0904.JPG No edit summary current

2 May 2011

• 11:5711:57, 2 May 2011 diff hist +479 N RunBlue Electrophoresis Buffer Created page with "'''Tris-Tricine SDS-PAGE Buffer for RUN-Blue Gel''' RunBlue SDS Running Buffer( cat #: NXB50500)<br>20x Buffer<br>* 0.8 M Tricine<br>* 1.2 M Tris<br>* 2% SDS<br>* 50 mM Sodium B..." current

1 May 2011

• 18:3418:34, 1 May 2011 diff hist +400 N ELISA Coating Buffer Created page with "'''Bicarbonate/carbonate coating buffer (100 mM)'''<br> 3.03 g Na2CO3,<br>6.0 g NaHCO3<br>1000 ml distilled water<br>pH 9.6, <br>'''PBS:'''<br>1.16 g Na2HPO4,<br>0.1 g KCl,<br..." current

28 April 2011

• 18:1018:10, 28 April 2011 diff hist 0 N File:Vp16 purification2 4 28 2011.jpg No edit summary current
• 17:5317:53, 28 April 2011 diff hist 0 N File:Vp16 purification 4 28 11.jpg No edit summary current
• 11:2011:20, 28 April 2011 diff hist +910 Transfection reagents comparison 8 No edit summary current
• 11:1911:19, 28 April 2011 diff hist 0 N File:Tr7 tr8 tr9 4 28 2011.JPG No edit summary current
• 11:1611:16, 28 April 2011 diff hist +497 Transfection reagents comparison 10 No edit summary current