User contributions for Paul.xu

08:3508:35, 7 October 2019 diff hist +854 N Chicken Antibody Cloning Created page with ". Antibody Library Construction Total RNAs were extracted from the spleens of hyperimmunized chickens to synthesize cDNA copies. The copies were used as templates to amplify t..." current

15:5915:59, 30 September 2019 diff hist +2,095 N Ni-NTA-Cy3 Created page with "(Ni2+-NTA)2-Cy3 Synthesis Ni2+-loaded, bis-NTA-Cy3 ((Ni2+-NTA)2-Cy3) was synthesized using a modification of the method described in [17]. Nα,Nα-bis (carboxymethyl)-L-lysine..." current
13:4713:47, 30 September 2019 diff hist 0 N File:2624 digestion.JPG No edit summary current
13:4613:46, 30 September 2019 diff hist 0 N File:2624 cloning.JPG No edit summary current
13:4613:46, 30 September 2019 diff hist +2,913 2621 No edit summary current
13:4513:45, 30 September 2019 diff hist +2,849 2615 No edit summary current
13:4313:43, 30 September 2019 diff hist 0 N File:VLP expression screening 9 28 19 2.jpg No edit summary current

22:0522:05, 23 September 2019 diff hist 0 N File:Protein exp screening 9 23 19 2.jpg No edit summary current
12:5012:50, 23 September 2019 diff hist 0 N File:Protein exp screening 9 23 19.jpg No edit summary current
09:3709:37, 23 September 2019 diff hist +26,714 Tasks for This Week No edit summary current
09:3009:30, 23 September 2019 diff hist +393 N Sybr Green Mix Created page with "10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20 SYBR-G 1:1000 2.5 µl 1U <br/>Hotstar Taq 0...." current

12:0212:02, 20 September 2019 diff hist 0 N File:2616 2615 cloning.JPG No edit summary current
12:0212:02, 20 September 2019 diff hist 0 N File:2619 Vector digestion.JPG No edit summary current
12:0112:01, 20 September 2019 diff hist 0 N File:2619 PCR2.JPG No edit summary current
12:0112:01, 20 September 2019 diff hist 0 N File:2619 PCR1.JPG No edit summary current
12:0112:01, 20 September 2019 diff hist 0 N File:2619 digestion2.JPG No edit summary current
12:0112:01, 20 September 2019 diff hist 0 N File:2619 digestion1.JPG No edit summary current
10:3010:30, 20 September 2019 diff hist +2,530 N Liposome Protocol Created page with "Liposome Extrusion Protocol Day 1 1. Prepare a starting concentration of 50 mM of lipids (e.g. 1:1 DOPC:DSPC + 30% cholesterol + 5% N-dod-DOPE). 2. Use the rotary evap..." current

17:3317:33, 19 September 2019 diff hist +10,936 Secretion Expression E coli No edit summary current
13:1913:19, 19 September 2019 diff hist +622 N Antibody Design Created page with "IEDB method PIGS in B-cell epitope prediction http://tools.iedb.org/pigs/ The protein 3D structure prediction methods predicted helices at the places which were shown to..." current

15:3215:32, 13 September 2019 diff hist +23,270 NGS Library Preparation No edit summary current
10:3810:38, 13 September 2019 diff hist +17,291 N Refolding by Dialysis Created page with "1 M urea and sonicated four times for 50 s at 70% of the maximum power in an ultrasonic homogenizer 4710 (Cole-Parmer Instrument Co.; Chicago, IL, USA), followed by centrifuga..." current

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