Subcloning Tasks

	ID	Name	DNA Size	Vector	Parent	Method	Date	Enzyme	Expression	Note
0	3161	pET28b-GST--Myc-HA-Flag-V5	224	433 pDB.His.GST	3159 pMiniT2-1FRG-HA-VL-kappa			Nde1-Xho1
	3127	pET28b-qBeta-qBeta-6XHis				PCR qbeta and clone back into 3097		Nde1-HindIII	C-His tag	PCR product is in pT&A3, 997, sequenced.
	812	pET30b-phoA
	3074	pET30b-HA-avitag-BirA				in pJet1.2 is good, subclone into pET28b, no his tag, need to subclone into pT7T30-Avi-BirA (still no his tag)
	938	PNGase F,	1145	pET30b	Nde1-Xho1	pJet1.2, sequencing is good, need to subclone into pET30b				C-His result
	939	Tte-Exo III	780	pET30b	Nde1-Xho1	pJet1.2 sequencing is good, need to subclone into pET30b				C-His result
	942	VVN endonuclease	716	pET28b-pMBP	Nco1-Xho1	subclone from 942-3-PJET1-2				C-His result
	3122	Crm197, secreted	1743	pET30b	Nde1-Xho1	pJet1.2				C-His
	931	PAK16D ligase	2267	pET28b	Nde1-HindIII-Xho1	pJet1.2 fragment 2 is good fragment 1  is also good				N-His
	945	pT7A30-pMBP		pT7A3	Xba1-Nco1	PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL				result
	947	pT7A30-6His-pMBP								result
	949	pET28b-GST-sfGFP-Myc-HA-Flag-V5		433 546
	824	pET28b-VVTopo
	985	pCold-GST-3C-AP205-M13-g3p		2047  pCold-C6H	983 pMiniT2-973-2	insert: Nde1-Sal1, vector: Nde1-Xho1				Result
	984	pT7A3-6H-SBP-SUMO-3C
	986	pT7A3-6His-NLS-Cas9-NLS-6xHis			need to sequencing, expression screening					result