User contributions for Paul.xu

31 August 2011

• 13:5513:55, 31 August 2011 diff hist 0 N File:PKM2 Muts 8 31 2011.JPG No edit summary current

27 August 2011

• 15:4015:40, 27 August 2011 diff hist +36 N PLentiGP-PKA-Alpha moved PLentiGP-PKA-Alpha to 300 PLentiGP-PKA-Alpha current
• 15:3515:35, 27 August 2011 diff hist 0 N File:300 pLentiGP Gluc PKAalpha restriction digestion 8 27 2011.jpg No edit summary current
• 15:3515:35, 27 August 2011 diff hist 0 N File:300 pLentiGP-Gluc-PKAalpha PCR screening 8 26 2011.jpg No edit summary current

17 August 2011

• 15:2115:21, 17 August 2011 diff hist +446 Vector list No edit summary current

3 August 2011

• 18:2218:22, 3 August 2011 diff hist +720 Gel-Solubilization-Buffer No edit summary current
• 18:0618:06, 3 August 2011 diff hist +2,371 Improved-Home-Made-Plasmid-Mini-Prep No edit summary current

27 July 2011

• 11:3311:33, 27 July 2011 diff hist +959 N Lentivirus Purification Ultrafiltration Created page with "1. After PEG/NaCl precipitation and centrifugation, a white pellet should be visible. 2. Carefully decant the supernatant and add 1ml 5 ml PBS (or other buffer) to the centrifug..." current

26 July 2011

• 18:0618:06, 26 July 2011 diff hist +2,638 BL21Cells No edit summary current

18 July 2011

• 11:1011:10, 18 July 2011 diff hist 0 N File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 18 2011.jpg No edit summary current

17 July 2011

• 16:0016:00, 17 July 2011 diff hist +34 N PLenti-IRES-TFR-EGFP' moved PLenti-IRES-TFR-EGFP' to PLenti-IRES-TFR-EGFP current
• 16:0016:00, 17 July 2011 diff hist +1,407 m PLenti-IRES-TFR-EGFP moved PLenti-IRES-TFR-EGFP' to PLenti-IRES-TFR-EGFP current
• 15:5915:59, 17 July 2011 diff hist +12,913 PAAV-Mcs No edit summary current
• 15:1515:15, 17 July 2011 diff hist +1,442 PAAV-cre 256 No edit summary current
• 13:3113:31, 17 July 2011 diff hist +1,445 PAAV-SMN1 302 No edit summary current
• 12:4012:40, 17 July 2011 diff hist 0 N File:PAAV Cre 7 17 2011.JPG No edit summary current

16 July 2011

• 09:5609:56, 16 July 2011 diff hist 0 File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 16 2011.jpg uploaded a new version of "File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 16 2011.jpg" current
• 09:5409:54, 16 July 2011 diff hist 0 N File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 14 2011 1.jpg No edit summary current
• 09:0509:05, 16 July 2011 diff hist 0 File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 14 2011.jpg uploaded a new version of "File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 14 2011.jpg" current
• 09:0509:05, 16 July 2011 diff hist 0 N File:PHP-CBA-DSP-PEI conjugate transfection comparison 7 15 2011.jpg No edit summary current

15 July 2011

• 15:1515:15, 15 July 2011 diff hist 0 N File:App.JPG No edit summary current

14 July 2011

• 14:5114:51, 14 July 2011 diff hist 0 N File:AAV.JPG No edit summary current

12 July 2011

• 14:2514:25, 12 July 2011 diff hist +1,950 Synthesis CBA ABOL No edit summary current

11 July 2011

• 14:3714:37, 11 July 2011 diff hist +4,205 N MultiSite Gateway Cloning Created page with "MultiSite Gateway Cloning Protocol == 2-fragment recombination == Element 1 attB1, attB5r<br>Element 2 attB5, attB2 forward PCR primers: attB1 5'-GGGG-ACA-AGT-TTG-TAC-AAA-AA..." current
• 09:1809:18, 11 July 2011 diff hist +888 N PEG8000-PCR-Purification Created page with "Purify PCR product using PEG 8000 This protocol removes DNA less than 300 bp in size. 1. Add 75 ÎŒl of 1X TE, pH 8.0 to a 25 ÎŒl PCR amplification reaction containing your PC..." current

10 July 2011

• 18:2318:23, 10 July 2011 diff hist 0 N File:PLentiGP pLVGP EcoR1 Xho1 Nde1 Cre 2011 7 10.jpg No edit summary current
• 17:1017:10, 10 July 2011 diff hist 0 N File:Cre PCR 2011 7 10.jpg No edit summary current
• 17:0317:03, 10 July 2011 diff hist +137 EGFP Lentivirus QC No edit summary current

26 June 2011

• 07:2707:27, 26 June 2011 diff hist +533 N GeneExpresso QC Created page with "== GeneExpresso QC: Stability<br> == Three months at 4 oC.<br> Date: 6/25/2011<br> <br> Image:GeneExpressoMax_QC_6_26_2011.JPG<br> 1: GeneExpresso<br> 2. GeneExpresso M..." current
• 07:1907:19, 26 June 2011 diff hist 0 N File:GeneExpressoMax QC 6 26 2011.JPG No edit summary current

23 June 2011

• 13:3713:37, 23 June 2011 diff hist +976 N Site-Directed Mutagenesis Created page with "== Oligo Design and Synthesis == Synthesize two complimentary oligonucleotides containing the desired mutation, flanked by unmodified nucleotide sequence (10 to 15 bp). == PCR ..." current

22 June 2011

• 18:4318:43, 22 June 2011 diff hist +708 N Calcium phosphate transfection protocol2 Created page with "1. Detech cells with 0.05% tryp­sin–eth­yl­ene­di­amine­tet­ra­ace­tic acid (EDTA) treat­ment.<br> 2. Stop trys­i­ni­za­tion by DMEM–10% FCS.<br>3. Remove trys..." current

21 June 2011

• 12:2412:24, 21 June 2011 diff hist +351 To-order No edit summary current

19 June 2011

• 17:1417:14, 19 June 2011 diff hist 0 N File:Pka alpha purification qiagen column.jpg No edit summary current
• 16:1316:13, 19 June 2011 diff hist +233 22NT IRES No edit summary current
• 12:5012:50, 19 June 2011 diff hist +1,852 N Stable Clones by Retrovirus Created page with "= Generate retrovirus = 1. Transfect 293T cells (40-60% confluent) with 3 plasmids: 9 ÎŒg of retroviral plasmid (e.g. pBABE-ING2), 4.5 ÎŒg of GAG-POL plasmid and 4.5 ÎŒg of VSV..." current
• 11:5111:51, 19 June 2011 diff hist +3,243 Protein-Transfection No edit summary current

15 June 2011

• 08:4208:42, 15 June 2011 diff hist 0 N File:PLentiGP-PKAalpha 6 14 2011 2.JPG No edit summary current
• 08:4208:42, 15 June 2011 diff hist 0 N File:PlentiGP-PKAalpha6-14 2011.JPG No edit summary current

12 June 2011

• 12:3812:38, 12 June 2011 diff hist +516 N Site-directed-Mutagenesis Created page with "1 PCR: Add <br>100ng of template DNA<br>5 ÎŒl 10 X buffer<br>125ng of forward primer <br>125ng of reverse primer <br>1ÎŒL of dNTP mix<br>3ÎŒL of quick solution reagent<br>2.5U o..." current

11 June 2011

• 14:1414:14, 11 June 2011 diff hist +1,617 PKA FPLC-Purification No edit summary current

9 June 2011

• 12:2912:29, 9 June 2011 diff hist +657 Agarose-Gel-Electrophoresis No edit summary current

7 June 2011

• 12:1312:13, 7 June 2011 diff hist +662 PLVGP No edit summary current
• 12:0512:05, 7 June 2011 diff hist 0 N File:PLentiGP-PKA-alpha-pLVGP 6 7 2011.JPG No edit summary current

5 June 2011

• 14:2714:27, 5 June 2011 diff hist +1,653 PMBP-Kinase-Cloning No edit summary current
• 10:2510:25, 5 June 2011 diff hist +207 Mammalian cell lysis buffer No edit summary current

4 June 2011

• 22:3622:36, 4 June 2011 diff hist +2,979 EndoToxin-Free Maxi Plasmid-Prep No edit summary current
• 22:1222:12, 4 June 2011 diff hist +809 RT-PCR No edit summary current

3 June 2011

• 18:4618:46, 3 June 2011 diff hist +141 N Product Inquery Created page with "1. Pfizer Inc, 858-526-4923 a Chinese, ask RP-8 TEV, he wants to get TEV without His Tag. GST-TEV is good. 2011-6-3 Category:Customers" current
• 13:3513:35, 3 June 2011 diff hist +4,511 Antibody Purification No edit summary current