User contributions for Paul.xu

7 March 2012

• 12:2612:26, 7 March 2012 diff hist +6,039 Lentivirus Concentration Ultracentrifuge No edit summary current
• 11:0511:05, 7 March 2012 diff hist +946 N Plasmid GigaPrep using FPLC Created page with "1. Grow a single colony of bacteria in 5 ml LB. 2. Grow bacteria in a Wave Bioreactor or a Bioflo 3000 bioreactor. 3. Spin down bacteria. 4. Lyse bacteria using Alkaline SDS-N..." current

6 March 2012

• 17:0117:01, 6 March 2012 diff hist +1,570 HTNCre HIC Purification No edit summary current
• 10:4210:42, 6 March 2012 diff hist +580 Cre-Taq-PKA QC 2012-Feb No edit summary current
• 10:1810:18, 6 March 2012 diff hist +5,331 HTNCre Purification Feb 2012 No edit summary current
• 10:1410:14, 6 March 2012 diff hist 0 N File:Cre recombinase 2 17 2012.jpg No edit summary current

5 March 2012

• 16:4916:49, 5 March 2012 diff hist 0 N File:Mep Cre purification 3 5 2012.jpg No edit summary current
• 11:3211:32, 5 March 2012 diff hist +977 N HiTrap Desalting Created page with "To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor.<br>1. Equilibrate the column with  10 ml buffer<br>2. Apply the sampl..." current
• 11:1511:15, 5 March 2012 diff hist +5,133 Taq Purification March 2012 No edit summary current

4 March 2012

• 10:3010:30, 4 March 2012 diff hist +536 N TBS Created page with "== Recipe : 10X TBS == '''10X TBS''': 500 mM Tris.HCl, pH 7.4 and 1500 mM NaCl. 500 ml of 1 M Tris.Cl pH 7.4 <br> NaCl: 88 g ultrapure water to 1 L<br> == 1X TBS: <..." current

3 March 2012

• 19:0119:01, 3 March 2012 diff hist +10,749 N Common Acids Created page with "Molarity of Concentrated Reagents<br> With tabulated dilutions to make 1 Molar Solutions of common reagents {| cellspacing="1" cellpadding="2" border="1" bgcolor="#dddddd" |- |..." current
• 18:5018:50, 3 March 2012 diff hist +1,736 10 X PBS No edit summary current

2 March 2012

• 10:3510:35, 2 March 2012 diff hist 0 N File:1012+1010digest detect 3-1-2012.JPG No edit summary current
• 10:3510:35, 2 March 2012 diff hist 0 N File:1012+1010clone 2-28-2012.JPG No edit summary current

1 March 2012

• 00:0100:01, 1 March 2012 diff hist +2,700 N Cre HIC Purification Created page with "Hydrophobic Interaction Chromatography Hydrophobic interaction (HIC) chromatography is a powerful and often overlooked method of protein purification. This method separates pro..." current

28 February 2012

• 13:1413:14, 28 February 2012 diff hist +421 N Lentivirus Purification Heparin Affinity Chromatography Created page with "== Fractogel EMD Heparin (S) column<br> == <br> 1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD He..." current
• 11:0311:03, 28 February 2012 diff hist +1,683 N Lentivirus-Production-CGMP Created page with "=== 293T cells === 1. Thaw a vial of 293T cells and place them in a Nunc T-75 flask. 2. Passage cells every three to four days into Nunc T-500 flasks 3. Transfer cells to 10-..." current
• 10:3610:36, 28 February 2012 diff hist +1,071 Lentivirus Purification PEG + Q Membrane by FPLC for in vivo No edit summary current

27 February 2012

• 23:5123:51, 27 February 2012 diff hist +2,391 N Chaperones Created page with "Chaperones Image:Chaperone.jpg {| cellspacing="0" border="2" frame="VOID" rules="NONE" |- | width="30" height="17" bgcolor="#ffff99" | # | width="30" height="17" bgcolor..." current
• 14:3014:30, 27 February 2012 diff hist 0 N File:1002 clone 2-22-2012.JPG No edit summary current
• 12:1412:14, 27 February 2012 diff hist 0 N File:1002 digest detect 2-24-2012.JPG No edit summary current

25 February 2012

• 22:1822:18, 25 February 2012 diff hist +15,457 Bacerial Expression Strategy No edit summary current
• 13:0113:01, 25 February 2012 diff hist +10,683 N NEB Competent Cells Created page with "NEB Competent Cells {| cellspacing="0" cellpadding="1" border="0" class="tableborder" |- | nowrap="nowrap" colspan="17" | Cloning Strain Properties |- | nowrap="nowrap" bgcolor..." current

22 February 2012

• 09:3909:39, 22 February 2012 diff hist 0 N File:1009 digest detect 2-21-2012.JPG No edit summary current
• 09:3209:32, 22 February 2012 diff hist +136 Transfection reagents 2012-2-9 No edit summary current

21 February 2012

• 16:5316:53, 21 February 2012 diff hist +1,754 Transfection reagents freeze-thaw each 2 weeks No edit summary current
• 16:5316:53, 21 February 2012 diff hist 0 N File:15 weeks blue.jpg No edit summary current
• 16:5216:52, 21 February 2012 diff hist 0 N File:15 weeks.jpg No edit summary current
• 16:4916:49, 21 February 2012 diff hist 0 File:Other TR 2.14.2012.JPG uploaded a new version of "File:Other TR 2.14.2012.JPG" current
• 16:4816:48, 21 February 2012 diff hist +1,496 1008-pLVX-loxP-sfGFP-loxP No edit summary current
• 16:3416:34, 21 February 2012 diff hist 0 N File:12weeks blue.JPG No edit summary current
• 16:3316:33, 21 February 2012 diff hist 0 N File:12weeks.JPG No edit summary current
• 16:0416:04, 21 February 2012 diff hist +172 N IPTG Stock Created page with "1 M IPTG Dissolve 12 g of IPTG (Gold Biotechnology; Catalog #: I2481C) in 50mL of distilled H2O.<br>Aliquot to 1 ml, store at -20 oC.<br><br> Category:Buffer_Recipe" current
• 15:4515:45, 21 February 2012 diff hist +542 Lentivirus QC 2-9-2012 No edit summary current
• 15:4315:43, 21 February 2012 diff hist 0 N File:528 529 500ml infection 2.9..2012.jpg No edit summary current
• 15:3315:33, 21 February 2012 diff hist 0 N File:1008-6 transfection 1-21-2012.JPG No edit summary current
• 15:3315:33, 21 February 2012 diff hist 0 N File:1008-5 transfection 1-21-2012.JPG No edit summary current
• 15:3315:33, 21 February 2012 diff hist 0 N File:1008-4 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1008-3 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1008-2 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1007-8 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1007-7 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1007-6 transfection 1-21-2012.JPG No edit summary current
• 15:3215:32, 21 February 2012 diff hist 0 N File:1007-5 transfection 1-21-2012.JPG No edit summary current
• 15:3115:31, 21 February 2012 diff hist 0 N File:1007-4 transfection 1-21-2012.JPG No edit summary current
• 15:3115:31, 21 February 2012 diff hist 0 N File:1007-3 transfection 1-21-2012.JPG No edit summary current
• 15:3115:31, 21 February 2012 diff hist 0 N File:1007-2 transfection 1-21-2012.JPG No edit summary current
• 15:3115:31, 21 February 2012 diff hist 0 N File:1007-1 transfection 1-21-2012.JPG No edit summary current

20 February 2012

• 00:1000:10, 20 February 2012 diff hist +2,077 Adenovirus Prestock No edit summary current

19 February 2012

• 23:4523:45, 19 February 2012 diff hist +904 N Special:Badtitle/NS106:Cloning Form Created page with "<noinclude> This is the "Cloning Form" form. To create a page with this form, enter the page name below; if a page with that name already exists, you will be sent to a form to ed..." current