User contributions for Paul.xu

14:1814:18, 3 April 2012 diff hist +2,080 N Lentivirus Pricing Created page with "== Vector Packaging == http://www.chla.org/site/c.ipINKTOAJsG/b.6061589/k.98D1/Fee_Structure.htm We charge $1500 and $2500 for 2L a..." current

21:4421:44, 1 April 2012 diff hist +18,278 Integrase Purification No edit summary current
21:3021:30, 1 April 2012 diff hist +1,484 N Excisionase Purification Created page with "Expression and purification of His-tagged Xis and its derivatives. E. coli strains DH5␣, harboring derivatives of the pYL-XisHR plasmid, and BL21(DE3), with the pYL-XisHP..." current
14:1014:10, 1 April 2012 diff hist +995 N Vector Features Created page with "{| width="200" cellspacing="1" cellpadding="1" border="1" |- | ID | Vector | Alias | Type | Cloning Method | Antibiotic | Promotor/ Repressor | Tag<..." current
14:0414:04, 1 April 2012 diff hist +8,655 TEV Purification No edit summary current

22:3722:37, 31 March 2012 diff hist +7,391 N Invitrogen Competent Cells Created page with "{| width="100%" cellspacing="0" |- ! valign="top" | Product Name ! valign="top" | Genotype |- | valign="top" | BL21-AI™ | valign="top" | F– ompT hsdSB(rB–, mB–) gal d..." current

18:3018:30, 29 March 2012 diff hist 0 N File:Lambda PCR screening2 3 29 2012.jpg No edit summary current
18:2518:25, 29 March 2012 diff hist 0 N File:Lambda colony PCR screening1 3 29 2012.jpg No edit summary current
18:2418:24, 29 March 2012 diff hist 0 N File:Lambda PCR screening 3 29 2012.jpg No edit summary current
10:0010:00, 29 March 2012 diff hist +636 N Gateway-Vector Construction Created page with "1. After PCR digest Gateway cassette with enzymes 2. Ligate to vector of choice to generate destination vector. 3. Transform top10/DH5alpha, or STBL3, grow on LB/amp at 37 oC o..." current

17:3517:35, 27 March 2012 diff hist +610 1033-pGST-DNase1 No edit summary current
14:5514:55, 27 March 2012 diff hist 0 N File:1009+1017+1023+1034 clone 3-27-2012.JPG No edit summary current
13:3113:31, 27 March 2012 diff hist +141 N Taq Growth Medium Created page with "0.5% bactotryptone, 0.3% yeast extract and 0.2% NaCl, autoclave. Grow Taq at 68 oC Category:Buffer_Recipe" current

18:5018:50, 26 March 2012 diff hist +9,527 N Library Prep for NextGen Sequencing Created page with "= Isolation of genomic DNA from zebrafish larvae for sequencing libraries = == Preparing Embryos or Larvae == #Separate embryos or larvae (I like 100 d5) per 50mL conical t..." current
18:4518:45, 26 March 2012 diff hist +519 N Rapid Run Agarose Gel Buffer Created page with "20x RapidRun Agarose Buffer *1.78 M Tris *0.57 M Taurine *0.01M EDTA adjust pH to 8.9, filter. Reagent Supplier purity Cat. no. approx. price Taurine (125.15 g/mol) Sig..." current
16:1216:12, 26 March 2012 diff hist 0 N File:1034 PCR 3-26-2012.JPG No edit summary current
16:0616:06, 26 March 2012 diff hist 0 File:I025 digest 3-26-2012.JPG uploaded a new version of "File:I025 digest 3-26-2012.JPG" current

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