User contributions for Paul.xu

Results for Paul.xu talk block log uploads logs

A user with 13,447 edits. Account created on 25 October 2010.

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11:3011:30, 25 April 2012 diff hist 0 N File:1023 subclone 4-25-2012.JPG No edit summary current

15:0215:02, 23 April 2012 diff hist 0 N File:1080 clone 4-23-2012.JPG No edit summary current
15:0215:02, 23 April 2012 diff hist 0 N File:1090 digest detect 4-23-2012.JPG No edit summary current

22:3622:36, 22 April 2012 diff hist +577 N QPCR Created page with "10x Platinum taq buffer (Invitrogen) 2.5μl <br>50mM MgCl 2 (→ 1.5mM) 0.75μl <br>template DNA 2.5μl <br>10μM DLP (→ 250nM) 0.625μl <br>50x R..." current

16:1116:11, 20 April 2012 diff hist +1,191 1079-pLVX-loxP-sfGFP-loxP-Gluc No edit summary current
15:2915:29, 20 April 2012 diff hist 0 N File:1080 PCR 2012-4-20.JPG No edit summary current
14:4914:49, 20 April 2012 diff hist 0 N File:1090 clone 4-20-2012-2.JPG No edit summary current
14:3414:34, 20 April 2012 diff hist 0 N File:1017 clone 4-20-2012.JPG No edit summary current
13:3113:31, 20 April 2012 diff hist +1,060 495-pAd52-CMV5-CuO-MCS No edit summary current
13:2913:29, 20 April 2012 diff hist +5,638 534-pLVX-EF1-IRES-EGFP No edit summary current
13:2713:27, 20 April 2012 diff hist 0 File:534 digest detect 4-20-2012.JPG uploaded a new version of "File:534 digest detect 4-20-2012.JPG" current
13:2513:25, 20 April 2012 diff hist 0 N File:495 digest detect 4-20-2012.JPG No edit summary current

14:1914:19, 19 April 2012 diff hist 0 N File:495 diges detect 4-19-2012.JPG No edit summary current
14:1914:19, 19 April 2012 diff hist 0 N File:1092+1095 digest detect 4-19-2012.JPG No edit summary current
14:1814:18, 19 April 2012 diff hist 0 N File:495+1092 digest detect 4-19-2012.JPG No edit summary current

16:4116:41, 18 April 2012 diff hist 0 N File:205 digestion 4 18 2012.jpg No edit summary current
15:2215:22, 18 April 2012 diff hist 0 N File:534 clone 4-18-2012.JPG No edit summary current
10:0910:09, 18 April 2012 diff hist 0 N File:495 clone 4-17-2012.JPG No edit summary current
10:0910:09, 18 April 2012 diff hist 0 N File:1092 clone 4-17-2012.JPG No edit summary current
10:0810:08, 18 April 2012 diff hist 0 N File:534 PCR 4-17-2012.JPG No edit summary current

22:3522:35, 17 April 2012 diff hist 0 N File:1063 digestion 4 17 2012.jpg No edit summary current
19:0619:06, 17 April 2012 diff hist 0 N File:PCR gateway cassett 4 17 2012.jpg No edit summary current
15:4415:44, 17 April 2012 diff hist +2,790 1030-pHis-GST-TEV No edit summary current
15:4215:42, 17 April 2012 diff hist 0 N File:GST-TEV 4 17 2012 2.jpg No edit summary current
15:1815:18, 17 April 2012 diff hist 0 N File:GST-TEV 4 17 2012.jpg No edit summary current

15:3915:39, 16 April 2012 diff hist 0 N File:537+1095 clone 4-16-2012.JPG No edit summary current
15:3915:39, 16 April 2012 diff hist 0 N File:537 clone 1st digest 4-13-2012.JPG No edit summary current
13:1913:19, 16 April 2012 diff hist 0 N File:1074 digest detect 4-16-2012.JPG No edit summary current
12:5512:55, 16 April 2012 diff hist +1,981 N Baculovirus Generation and Amplificaiton Created page with "== Generation of Baculovirus == 1. Subclone gene into pFastBac-DUAL<br>2. Select and isolate the recombinant donor plasmid.<br>3. Transform the recombinant donor plasmid into DH..." current
12:2012:20, 16 April 2012 diff hist +2,770 N Lentivirus Titering Created page with "HIV-PSI F 5 -ACCTGAAAGCGAAAGGGAAAC<br>HIV-PSI R 5 -CGCACCCATCTCTCTCCTTCT<br>HIV-PSI-FAM 5 -6-FAM-AGCTCTCTC-ZEN-GACGCAGGACTCGGC-Iowa Black FQ<br> SDC4 F 5 -CAGGGTCTGGGAGCCAAGT<br..." current
12:1412:14, 16 April 2012 diff hist +2,125 N Lentivirus Concentration by Ultrafiltration Created page with "1. Grow low passage (<12) 293T cells below confluence in 500 cm2 TripleFlasks in 150 mL D10 medium (DMEM with 10% fetal bovine serum, 50 U/mL penicillin,50 ug/mL streptomycin..." current
11:3711:37, 16 April 2012 diff hist 0 N File:1017+1095 PCR 4-13-2012.JPG No edit summary current
11:3711:37, 16 April 2012 diff hist 0 N File:1074 clone 4-13-2012.JPG No edit summary current
11:3611:36, 16 April 2012 diff hist 0 N File:1079 digest detect 4-13-2012.JPG No edit summary current

14:3614:36, 15 April 2012 diff hist +5,361 Adenovirus Plaque Assay No edit summary current
13:5613:56, 15 April 2012 diff hist +2,673 N AAV Purification by Chromatography Created page with "1. Harvest adherent cells into serum-free medium by gentle scraping. Most laboratory-scale preps (<10^9 cells) can be harvested into a total pooled volume of 200 ml or less. C..." current

16:4416:44, 14 April 2012 diff hist +258 Adenovirus Production 293F No edit summary current

15:1015:10, 12 April 2012 diff hist +1,550 1011-pET30b-FluASeg-7 No edit summary current
15:0915:09, 12 April 2012 diff hist +157 Taq Muts Purification No edit summary current
15:0715:07, 12 April 2012 diff hist 0 N File:1011 ET30b-FluASeg-7 expression.jpg No edit summary current
15:0615:06, 12 April 2012 diff hist 0 N File:Taq muts purification 4 12 2012.jpg No edit summary current
14:4314:43, 12 April 2012 diff hist 0 N File:406-pET28b-Int-T7.ab1 No edit summary current
14:4114:41, 12 April 2012 diff hist 0 N File:406-pET28b-Int-T7 good.seq No edit summary current

13:5213:52, 11 April 2012 diff hist 0 N File:1079 clone 4-11-2012.JPG No edit summary current
11:4411:44, 11 April 2012 diff hist 0 File:203 turboTEV gene syn 4 11 2012.jpg uploaded a new version of "File:203 turboTEV gene syn 4 11 2012.jpg" current

16:3116:31, 10 April 2012 diff hist 0 N File:Phi29 Gibson assembly 4 10 2012.jpg No edit summary current
15:4715:47, 10 April 2012 diff hist 0 N File:1079 PCR 4-10-2012.JPG No edit summary current
13:4413:44, 10 April 2012 diff hist +1,398 1022-pET28b-Tth-Ligase No edit summary current
13:4313:43, 10 April 2012 diff hist +3,972 1048-pET30b-RNaseI No edit summary current
13:4113:41, 10 April 2012 diff hist 0 N File:1022+1023 digest detect 4-10-2012.JPG No edit summary current

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