User contributions for Paul.xu
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28 February 2014
- 15:3315:33, 28 February 2014 diff hist 0 N File:2015 2076pcr 2-21-2014.JPG No edit summary current
- 15:3015:30, 28 February 2014 diff hist +757 N 729-pLysN-mEcoR1 Created page with "== '''Cloning of 729-pLysN-mEcoR1''' == '''Purpose:''' Cloning of 729-pLysN-mEcoR1 '''Date: '''2014-2-25---2014-2-27 '''Notebook:0''' '''Page:''' '''Clone ID:729''' ''..." current
- 15:2715:27, 28 February 2014 diff hist +757 N 728-pLysN-mBamH1 Created page with "== '''Cloning of 728-pLysN-mBamH1''' == '''Purpose:''' Cloning of 728-pLysN-mBamH1 '''Date: '''2014-2-25---2014-2-27 '''Notebook:0''' '''Page:''' '''Clone ID:728''' '''..." current
- 15:2515:25, 28 February 2014 diff hist 0 N File:728 729digest 2-27-2014.JPG No edit summary current
- 15:2515:25, 28 February 2014 diff hist 0 N File:728 729clone 2-25-2014.JPG No edit summary current
- 15:2115:21, 28 February 2014 diff hist +841 N 353-pT7A3E-HC-TaqMuts Created page with "== '''Cloning of 353-pT7A3E-HC-TaqMuts''' == '''Purpose:''' Cloning of 353-pT7A3E-HC-TaqMuts '''Date: '''2014-2-26---2014-2-28 '''Notebook:0''' '''Page:''' '''Clone ID:35..." current
- 15:1915:19, 28 February 2014 diff hist 0 N File:2057digest1 2-28-2014.JPG No edit summary current
- 15:1915:19, 28 February 2014 diff hist 0 N File:353digest 2-28-2014.JPG No edit summary current
- 15:1815:18, 28 February 2014 diff hist 0 N File:353 2033 2057clone 2-26-2014.JPG No edit summary current
- 15:1815:18, 28 February 2014 diff hist 0 N File:353 2033 2057PCR 2-26-2014.JPG No edit summary current
- 10:3610:36, 28 February 2014 diff hist 0 N File:2080clone 2-24-2014.JPG No edit summary current
- 10:3110:31, 28 February 2014 diff hist 0 N File:2040clone 2-24-2014.JPG No edit summary current
25 February 2014
- 15:5715:57, 25 February 2014 diff hist +636 1519-pT7A3-T3-DNA-Ligase No edit summary current
- 15:5115:51, 25 February 2014 diff hist 0 N File:2014clone 2-24-2014.JPG No edit summary current
- 15:5115:51, 25 February 2014 diff hist 0 N File:2012 2014 2015 2076 2078PCR 2-24-2014.JPG No edit summary current
- 15:2415:24, 25 February 2014 diff hist +1,853 Plasmid Miniprep with Vacuum No edit summary current
- 14:0014:00, 25 February 2014 diff hist +1,896 N Taq Muts Created page with "== Purification of Taq-Muts, no tag<br> == 1. Grow E. coli strain such as BL21(DE3) transformed with 353 pT7A3E-HC-TaqMuts in 8L LB/AMP at 37 °C.<br>2. Induce for 4 h with 1 mM..." current
24 February 2014
- 15:3715:37, 24 February 2014 diff hist +860 2074-pET28b-Penetratin-Cre No edit summary current
- 15:3615:36, 24 February 2014 diff hist 0 N File:2074digest 2-24-2014.JPG No edit summary current
- 15:2315:23, 24 February 2014 diff hist +174 N 3C digestion Created page with "Use 1 mg 3C protease to digest 100 mg fusion protein.<br> Buffer<br> 50 mM Tris-HCl, 150 mM NaCl, 1 mM dithiothreitol (DTT), pH 7.0 ~8.0.<br> Category:Protocols" current
23 February 2014
- 18:5918:59, 23 February 2014 diff hist +765 N 2096 2097 Created page with "Date: 2014/2/20<br> Cells: BL21-AI, 5 ml, induce with 0.15 mM o/n at 18 oC. (cell initially all lysed, only a few survived, then growed to OD600 ~=1 at 37 oC, cool down to ~20 o..." current
- 18:4818:48, 23 February 2014 diff hist 0 N File:2096 2097 expression screening.jpg No edit summary current
22 February 2014
- 17:5617:56, 22 February 2014 diff hist 0 File:TR comp 2014 2 22 2.JPG uploaded a new version of "File:TR comp 2014 2 22 2.JPG" current
- 16:4316:43, 22 February 2014 diff hist 0 N File:TR comp 2014 2 22 1.JPG No edit summary current
21 February 2014
- 15:0815:08, 21 February 2014 diff hist +844 N 2079-pLVX-p53 Created page with "== '''Cloning of 2079-pLVX-p53''' == '''Purpose:''' Cloning of 2079-pLVX-p53 '''Date: '''2014-2-19---2014-2-21 '''Notebook:0''' '''Page:''' '''Clone ID:2079''' '''Exper..." current
- 15:0315:03, 21 February 2014 diff hist +1,976 1940-pLysN-ha-Plac-Hia5 No edit summary current
- 15:0215:02, 21 February 2014 diff hist 0 N File:1940pcr screen 2-21-2014.JPG No edit summary current
- 15:0115:01, 21 February 2014 diff hist 0 N File:2074clone 2-21-2014.JPG No edit summary current
- 15:0115:01, 21 February 2014 diff hist 0 N File:2079digest2-21-2014.JPG No edit summary current
- 15:0115:01, 21 February 2014 diff hist 0 N File:2074 2079clone 2-19-2014.JPG No edit summary current
- 15:0115:01, 21 February 2014 diff hist 0 N File:2079PCR 2-19-2014.JPG No edit summary current
- 12:5612:56, 21 February 2014 diff hist +843 N 2056-pIC-TagRFP Created page with "== '''Cloning of 2056-pIC-TagRFP''' == '''Purpose:''' Cloning of 2056-pIC-TagRFP '''Date: '''2014-2-18--2014-2-20 '''Notebook:0''' '''Page:''' '''Clone ID:2056''' '''E..." current
- 12:5312:53, 21 February 2014 diff hist +1,002 N 1146-pT7A3E-Alk-PPtase Created page with "== '''Cloning of 1146-pT7A3E-Alk-PPtase''' == '''Purpose:''' Cloning of 1146-pT7A3E-Alk-PPtase '''Date: '''2014-2-17---2014-2-20 '''Notebook:0''' '''Page:''' '''Clone ID:..." current
- 12:4512:45, 21 February 2014 diff hist +1,075 N 1981-pCMV5-Tat-Flp-PDGFR Created page with "== '''Cloning of 1981-pCMV5-Tat-Flp-PDGFR''' == '''Purpose:''' Cloning of 1981-pCMV5-Tat-Flp-PDGFR '''Date: '''2014-2-18--2014-2-20 '''Notebook:0''' '''Page:''' '''Clone..." current
- 12:3812:38, 21 February 2014 diff hist 0 N File:2056digest 2-20-2014.JPG No edit summary current
- 12:3812:38, 21 February 2014 diff hist 0 N File:1146 1981digest 2-20-2014.JPG No edit summary current
- 12:3812:38, 21 February 2014 diff hist 0 N File:1146 1981 2012 2056clone 2-18-2014.JPG No edit summary current
- 12:3812:38, 21 February 2014 diff hist 0 N File:1146pcr 2-12-2014.JPG No edit summary current
- 12:3812:38, 21 February 2014 diff hist 0 N File:1146 2012 2045 2056PCR 2-14-2014.JPG No edit summary current
- 11:1511:15, 21 February 2014 diff hist +716 2000 pT7A3-8H-E coli Endo IV No edit summary current
20 February 2014
- 18:3318:33, 20 February 2014 diff hist +2,527 N Cell Adaptation Protocol Created page with "= Cell Adaptation Protocols<br> = == Introduction == There are two approaches to be considered when adapting cells to Sf-900 II SFM: 1) Direct planting of cells from medium con..." current
- 17:1117:11, 20 February 2014 diff hist +679 2091 pT7A3-MBP-Tat-NLS-yFlp-6H No edit summary current
- 17:1117:11, 20 February 2014 diff hist +681 N 2090 pT7A3-MBP-Tat-Flp-ecoli-6H Created page with "== '''Cloning of 2090 pT7A3-MBP-Tat-Flp-ecoli-6H''' == '''Purpose:''' Cloning of2090 pT7A3-MBP-Tat-Flp-ecoli-6H'''==''' '''Date: '''2014-2---- '''Notebook:0''' '''Page:''..." current
- 17:0717:07, 20 February 2014 diff hist 0 N File:2090digest.JPG No edit summary current
- 17:0617:06, 20 February 2014 diff hist 0 N File:2091digest.JPG No edit summary current
- 16:5816:58, 20 February 2014 diff hist 0 N File:DIGEST FOR 2062.JPG No edit summary current
- 16:5816:58, 20 February 2014 diff hist 0 File:2062 2028pcr.JPG uploaded a new version of "File:2062 2028pcr.JPG" current
- 16:5516:55, 20 February 2014 diff hist +890 N 2063 pT7A3E-ChiBD-6H Created page with "== '''Cloning of 2063 pT7A3E-ChiBD-6H''' == '''Purpose:''' Cloning of2063 pT7A3E-ChiBD-6H'''==''' '''Date: '''2014-2---- '''Notebook:0''' '''Page:''' '''Clone ID:2063''..." current
- 16:5516:55, 20 February 2014 diff hist 0 N File:2062 2063DIGEST.JPG No edit summary current
- 16:4616:46, 20 February 2014 diff hist 0 N File:2050 2055 2063 2065 2070 2064pcr.JPG No edit summary current
