User contributions for Paul.xu

12 May 2014

• 16:1516:15, 12 May 2014 diff hist +252 N Error Removal by T7 Enonuclease I Created page with "1. After PCR, purify PCR product using spin column, elute with 20 ul water 2. Add 15 units of T7 endonuclease I,  2 ul of 10 X T7 endonuclease I buffer. 3. Incubate at 3..." current
• 12:4412:44, 12 May 2014 diff hist 0 N File:2180digest5-12-214.JPG No edit summary current
• 11:2511:25, 12 May 2014 diff hist 0 N File:Cre activity assay 20140509F.jpg No edit summary current
• 11:1711:17, 12 May 2014 diff hist 0 N File:Cre activity assay 20140509DONE.jpg No edit summary current

9 May 2014

• 17:4517:45, 9 May 2014 diff hist +7,019 N Baculovirus Expression Created page with "== Preparing and titering baculovirus working stocks for protein production. == <br>Because we can only send you a small sample of a plaque-purified, first passage (PP1P1) virus..." current
• 17:3417:34, 9 May 2014 diff hist +8,836 N 293F Expression Created page with "== Transient Transfection of HEK-293F Suspension Cultures using PEI == Cells are grown in suspension on a platform shaker in a humidified 37°C CO2 incubator with rotation at ~1..." current
• 17:0417:04, 9 May 2014 diff hist 0 N File:2178 2179 2184 2082 2086 2087 2088.jpg No edit summary current
• 17:0417:04, 9 May 2014 diff hist 0 N File:2107 1836 2038 2040 2080.jpg No edit summary current
• 12:2612:26, 9 May 2014 diff hist +887 2194-pT7A3E-HC-Tth-End-IV No edit summary current
• 12:2312:23, 9 May 2014 diff hist 0 N File:2194digest2 5-9-2014.JPG No edit summary current
• 12:2312:23, 9 May 2014 diff hist 0 N File:2194digest1 5-9-2014.JPG No edit summary current
• 12:0912:09, 9 May 2014 diff hist +760 N 2077-pEC-R1-CopB-RepA Created page with "== '''Cloning of 2077-pEC-R1-CopB-RepA''' == '''Purpose:''' Cloning of 2077-pEC-R1-CopB-RepA '''Date: '''2014-5-9 '''Notebook:''' '''Page:''' '''Clone ID:2077''' '''Exp..." current
• 12:0212:02, 9 May 2014 diff hist 0 N File:2077pcr1 5-9-2014.JPG No edit summary current
• 11:5911:59, 9 May 2014 diff hist 0 N File:2194clone 5-7-2014.JPG No edit summary current
• 11:5711:57, 9 May 2014 diff hist +2,049 2156-pJ912-HIS4 No edit summary current
• 11:5511:55, 9 May 2014 diff hist 0 N File:2156-3pcr screen 5-9-2014.JPG No edit summary current

8 May 2014

• 15:2815:28, 8 May 2014 diff hist +762 N 2085-pT7A3-MBP-TRX-sso7d-6H Created page with "== '''Cloning of 2085-pT7A3-MBP-TRX-sso7d-6H''' == '''Purpose:''' Cloning of 2085-pT7A3-MBP-TRX-sso7d-6H '''Date: '''2014-5-6---2014-5-8 '''Notebook:0''' '''Page:''' '''..." current
• 15:2615:26, 8 May 2014 diff hist 0 N File:2049 2012 2085clone 5-6-2014.JPG No edit summary current
• 15:2415:24, 8 May 2014 diff hist +841 2195-pT7A3E-Vac-DNA-Ligase No edit summary current
• 15:2315:23, 8 May 2014 diff hist 0 N File:2196digest 5-8-2014.JPG No edit summary current
• 15:2315:23, 8 May 2014 diff hist 0 N File:2085 2195digest 5-8-2014.JPG No edit summary current

7 May 2014

• 14:2314:23, 7 May 2014 diff hist +827 2163-pT7A3E-HC-Tma-Endo-IV No edit summary current
• 14:1714:17, 7 May 2014 diff hist 0 N File:2156clone 5-7-2014.JPG No edit summary current
• 14:1714:17, 7 May 2014 diff hist 0 N File:2156 2194pcr 5-7-2014.JPG No edit summary current
• 14:1414:14, 7 May 2014 diff hist 0 N File:2156digest 5-7-2014.JPG No edit summary current
• 12:1012:10, 7 May 2014 diff hist +12,352 Magnetic Nanoparticles No edit summary current
• 10:3010:30, 7 May 2014 diff hist +1,785 RNase A Purification No edit summary current

6 May 2014

• 23:2323:23, 6 May 2014 diff hist +4,812 Gateway Enzymes →LR4 buffer (5x) - for use with attL1.1 and attL2.1 current
• 15:3615:36, 6 May 2014 diff hist 0 N File:2195 2196clone 5-6-2014.JPG No edit summary current
• 15:3615:36, 6 May 2014 diff hist 0 N File:2049 2012 2195PCR 5-6-2014.JPG No edit summary current

5 May 2014

• 15:5515:55, 5 May 2014 diff hist +1,032 N 2185-pCold-MBP-(Ent-TEV) Created page with "== '''Cloning of 2185-pCold-MBP-(Ent-TEV)''' == '''Purpose:''' Cloning of 2185-pCold-MBP-(Ent-TEV) '''Date: '''2014-5-2---2014-5-5 '''Notebook:0''' '''Page:''' '''Clone I..." current
• 15:5115:51, 5 May 2014 diff hist +746 N 2187-pT7A3HC-6H-HA2-R9 Created page with "== '''Cloning of 2187-pT7A3HC-6H-HA2-R9''' == '''Purpose:''' Cloning of 2187-pT7A3HC-6H-HA2-R9 '''Date: '''2014-5-2---2014-5-5 '''Notebook:0''' '''Page:''' '''Clone ID:21..." current
• 15:4815:48, 5 May 2014 diff hist 0 N File:2185 2187digest 5-5-2014.JPG No edit summary current
• 11:5311:53, 5 May 2014 diff hist +7,753 N Protein Purification using Magnetic Beads Created page with "= His-tag protein purification using Ni-NTA magnetic beads<br><br> = == Buffers == '''Extraction buffer:''' 20 mM Tris-HCl, pH 8.0<br> 1 mM PMSF<br> 1 mg/ml lysozyme<br> 0.05%..." current

4 May 2014

• 17:1817:18, 4 May 2014 diff hist +650 N 2184 pMAL-c5E-R9-HA-iFlp Created page with "== '''Cloning of 2184 pMAL-c5E-R9-HA-iFlp''' == '''Purpose:''' Cloning of2184 pMAL-c5E-R9-HA-iFlp'''==''' '''Date: '''2014-4---- '''Notebook:0''' '''Page:''' '''Clone I..." current
• 17:1617:16, 4 May 2014 diff hist +666 N 2179 pT7A3HC-6H-Tat-sc-MS2-Flpo Created page with "== '''Cloning of 2179 pT7A3HC-6H-Tat-sc-MS2-Flpo''' == '''Purpose:''' Cloning of2179 pT7A3HC-6H-Tat-sc-MS2-Flpo'''==''' '''Date: '''2014-4---- '''Notebook:0''' '''Page:''..." current
• 17:1517:15, 4 May 2014 diff hist +668 N 2178 pT7A3HC-6H-Tat-sc-MS2-iCre Created page with "== '''Cloning of 2178 pT7A3HC-6H-Tat-sc-MS2-iCre''' == '''Purpose:''' Cloning of2178 pT7A3HC-6H-Tat-sc-MS2-iCre'''==''' '''Date: '''2014-4---- '''Notebook:0''' '''Page:''..." current
• 17:1417:14, 4 May 2014 diff hist 0 N File:2178 2179 2184digest.JPG No edit summary current
• 17:1417:14, 4 May 2014 diff hist 0 N File:Digest for 2178 2179 2184.JPG No edit summary current
• 16:1916:19, 4 May 2014 diff hist +665 2154 pCMV5-Gag-Cre-IRES-VSVG-WPRE-oriP No edit summary current

30 April 2014

• 15:4615:46, 30 April 2014 diff hist +852 N 2190-pT7A3E-6H-Tth-SOD Created page with "== '''Cloning of 2190-pT7A3E-6H-Tth-SOD''' == '''Purpose:''' Cloning of 2190-pT7A3E-6H-Tth-SOD '''Date: '''2014-4-24---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone ID:..." current
• 15:4415:44, 30 April 2014 diff hist +985 2189-pEC2-Tth-SOD No edit summary current
• 15:0015:00, 30 April 2014 diff hist +815 N 2188-pT7A3E-6H-HC-Tat-HA Created page with "== '''Cloning of 2188-pT7A3E-6H-HC-Tat-HA''' == '''Purpose:''' Cloning of 2188-pT7A3E-6H-HC-Tat-HA '''Date: '''2014-4-24---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone..." current
• 14:4214:42, 30 April 2014 diff hist +917 N 2169-pPIC9k-Enterokinase Created page with "== '''Cloning of 2169-pPIC9k-Enterokinase''' == '''Purpose:''' Cloning of 2169-pPIC9k-Enterokinase '''Date: '''2014-4-24---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone..." current
• 14:3614:36, 30 April 2014 diff hist +815 N 2101-pT7A3-sso7d Created page with "== '''Cloning of 2101-pT7A3-sso7d''' == '''Purpose:''' Cloning of 2101-pT7A3-sso7d '''Date: '''2014-4--26---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone ID:2101''' '..." current
• 14:3114:31, 30 April 2014 diff hist +836 N 2093-pT7A3-MBP-(TEV)-MBP-NusA-sso7d-6H Created page with "== '''Cloning of 2093-pT7A3-MBP-(TEV)-MBP-NusA-sso7d-6H''' == '''Purpose:''' Cloning of 2093-pT7A3-MBP-(TEV)-MBP-NusA-sso7d-6H '''Date: '''2014-4-24---2014-4-28 '''Notebook:0..." current
• 14:2314:23, 30 April 2014 diff hist 0 N File:2093 2163 2185pcr 4-24-2014.JPG No edit summary current
• 13:2813:28, 30 April 2014 diff hist 0 N File:2186 2188 2163 2189 2190PCR 4-25-2014.JPG No edit summary current
• 13:2813:28, 30 April 2014 diff hist 0 N File:2033 2093 2101 2169 2185PCR 4-25-2014.JPG No edit summary current
• 13:2713:27, 30 April 2014 diff hist 0 N File:2190clone 4-25-2014.JPG No edit summary current