User contributions for Crm

19:1419:14, 14 June 2017 diff hist +2,240 N MS 6 14 2017 Created page with "Spin down 2295 yPolyA. Resuspend 1483 T4 DNA Ligase, homogenize, load onto Ni column. Wash Ni column (bind with 1498 Taq DNA Ligase) with Ni washing buffer, Tris-HCl, Buffer..." current
19:1319:13, 14 June 2017 diff hist 0 N File:Protein Screening 1518 1601 1765 1942 06 14 2017.jpg No edit summary current
19:0619:06, 14 June 2017 diff hist 0 N File:Protein Expression SDS-PAGE 127-1946 TdT bovine QHP 6 14 2017.jpg No edit summary current
18:5718:57, 14 June 2017 diff hist +852 N MS 6 13 2017 Created page with "Culture 2295 yPolyA 8L, induced with IPTG. Spin down 1483 T4 DNA Ligase. Wash Ni column (bind with 1497 ToughTaq) with Ni washing buffer, Tris-HCl, Buffer B, dilute and load..." current
18:5618:56, 14 June 2017 diff hist 0 N File:128-1497 ToughTaq QHP 6 14 2017.jpg No edit summary current
18:5618:56, 14 June 2017 diff hist 0 N File:127-1946 TdT bovine QHP 6 13 2017.jpg No edit summary current
18:4118:41, 14 June 2017 diff hist +338 MS 6 12 2017 No edit summary current

16:4516:45, 5 June 2017 diff hist +801 N MS 6 5 2017 Created page with "Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column. Elute 483 Tth Polymerase with Buffer B 06/05/2017..." current

16:5916:59, 2 June 2017 diff hist +4,981 MS reports 5 30 2017-6 2 2017 No edit summary current
16:5816:58, 2 June 2017 diff hist +252 N MS 6 2 2017 Created page with "Regenerate Ni column and SP FF column. Elute 1782 Tth Ligase. File:124-1782 Tth Ligase QHP 6 2 2017.jpg Homogenize 483 Tth Polymerase, spin down, load supernatant onto..." current
16:5516:55, 2 June 2017 diff hist +407 MS 5 31 2017 No edit summary current
16:5416:54, 2 June 2017 diff hist 0 N File:124-1782 Tth Ligase QHP 6 2 2017.jpg No edit summary current
16:5416:54, 2 June 2017 diff hist 0 N File:123-1708 HTN Cre SP FF 5 31 2017.jpg No edit summary current

18:0018:00, 1 June 2017 diff hist +696 N MS 6 1 2017 Created page with "Culture 1708 HTN Cre (8L), induced with IPTG, over night. Spin down 483 Tth Polymerase. Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse. Culture 14..." current
14:3214:32, 1 June 2017 diff hist +3,603 MS 5 30 2017 No edit summary current

16:2516:25, 26 May 2017 diff hist +8,471 MS reports 5 23 2017-5 26 2017 No edit summary current
16:2316:23, 26 May 2017 diff hist +1,584 N MS 5 26 2017 Created page with "Prepare LB media (20L). Spin down 1708 HTNCre, wash with buffer A, break down through homogenizer, collect supernatant, load onto Ni column. Troubleshoot with printer, load..." current
16:1216:12, 26 May 2017 diff hist 0 N File:Protein Expression 483 1464 1946 1497 05 26 2017.jpg No edit summary current

17:4117:41, 25 May 2017 diff hist +2,141 N MS 5 25 2017 Created page with "Use IPTG to induce 483 Tth Polymerase /1464 T7 DNA Ligase / 1946 TdT bovine / 1497 ToughTaq for expression screening. Spin down cells, wash with Lysis Buffer, break down throu..." current
17:4017:40, 25 May 2017 diff hist 0 N File:Protein Screening 1613 1782 2196 1498 2295 05 25 2017.jpg No edit summary current

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