User contributions for Paul.xu

6 May 2018

• 19:3819:38, 6 May 2018 diff hist +32,134 Human Naive Library Construction No edit summary current

5 May 2018

• 21:3821:38, 5 May 2018 diff hist +16,354 N Construction of Large Naive Fab Library Created page with "<br/>Antibody Phage Display Methods and Protocols<br/>Edited by<br/>Philippa M. O’Brien<br/>and<br/>Robert Aitken<br/>University of Glasgow, Glasgow, Scotland, UK, 2002 Con..." current
• 20:3120:31, 5 May 2018 diff hist +18,516 Human scFV Library Construction Protocol No edit summary current

4 May 2018

• 14:4514:45, 4 May 2018 diff hist +233 N Gene synthesis Tasks Created page with "{| border="1" cellspacing="1" cellpadding="1" style="width:500px;" |- | ID | Name | <br/> | <br/> | <br/> |- | 1916<br/> | pMiniT2-M-SssI<br/> | <br/> | <br/> | <br/> |- | <br..." current

3 May 2018

• 16:5016:50, 3 May 2018 diff hist +6,920 Naive Mouse Antibody No edit summary current
• 14:1914:19, 3 May 2018 diff hist 0 N File:Mouse synthetic library.png No edit summary current
• 11:5111:51, 3 May 2018 diff hist +633 N SMARTer RACE Primers Created page with "The forward primers in the Nested Universal Primer A of the SMARTer RACE cDNA  Amplification Kit 5′–AAGCAGTGGTATCAACGCAGAGT–3′ Reverse primers: ..." current
• 11:5011:50, 3 May 2018 diff hist 0 N File:Template switching oligos.jpg No edit summary current
• 11:2111:21, 3 May 2018 diff hist +21,560 Tag Antibodies No edit summary current

2 May 2018

• 18:0318:03, 2 May 2018 diff hist +11,682 Construction of the human antibody gene libraries No edit summary current
• 17:4117:41, 2 May 2018 diff hist +1,178 N Humanization of the antibodies by germline-humanization Created page with "Humanization of the macaque antibodies by germline-humanization<br/>The sequence of each antibody was compared with the human germline genes using the<br/>IMGT/V-QUEST online..." current

1 May 2018

• 16:2116:21, 1 May 2018 diff hist +17,812 Oligos for cloning antibody No edit summary current

28 April 2018

• 10:0210:02, 28 April 2018 diff hist +42,604 Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly No edit summary current
• 10:0210:02, 28 April 2018 diff hist 0 N File:ScFv generation.png No edit summary current

27 April 2018

• 15:5815:58, 27 April 2018 diff hist +30,429 Human Phage Display Library No edit summary current

26 April 2018

• 16:2716:27, 26 April 2018 diff hist +241 N Mammalian Secretion Signals Created page with "CD47 <br/>atgtggcccctggtagcggcgctgttgctgggctcggcgGgctgcggatcCgct<br/> M  W  P  L  V  A  A  L  L  L  G  S ..." current

22 April 2018

• 15:2715:27, 22 April 2018 diff hist +7,017 Peptides for Transfection No edit summary current

21 April 2018

• 21:5121:51, 21 April 2018 diff hist +58,428 N Phage Display from Synthetic Library Created page with "=== Abstract === The demand for antibodies that fulfill the needs of both basic and clinical research applications is high and will dramatically increase in the future. Howev..." current
• 21:4821:48, 21 April 2018 diff hist +979 N Drug Targets for Antibodies Created page with "热门靶点对应抗体药物分析 统计的328已上市或在研抗体类药物靶点中，应用最广泛的是CD19,有64个药物以其为靶点，其中53个是CARs[7](..." current

20 April 2018

• 17:1417:14, 20 April 2018 diff hist +27,454 N List of Antibody Drugs Created page with "{| border="1" |- ! Antibody ! Company * ! Target ! Indication ! Source ! Approval * |- | 3F8 | Memorial Sloan-Kettering Merck | GD2 | Detection and treatmenf of neuroblastoma..." current

19 April 2018

• 19:0819:08, 19 April 2018 diff hist +737 944 pET30b-pfu-exo No edit summary current

18 April 2018

• 11:4811:48, 18 April 2018 diff hist +1,124 N Hybridoma Bioreactor Culture Created page with "Top chamber:<br/>DMEM<br/>https://www.thermofisher.com/order/catalog/product/11995065?SID=srch-hj-11995-065<br/> <br/>(5%) CDM HD serum replacement<br/>http://www.fiberce..." current

17 April 2018

• 17:4017:40, 17 April 2018 diff hist +6,080 Indirect ELISA No edit summary current

8 April 2018

• 09:5409:54, 8 April 2018 diff hist +7,579 Cloning VH and VL from hybridoma No edit summary current

7 April 2018

• 16:2316:23, 7 April 2018 diff hist +23 N Cloning Mouse VH and VL Created page with "Category:Antibodies" current

5 April 2018

• 22:5022:50, 5 April 2018 diff hist +2,722 TMB Solution No edit summary current
• 13:1913:19, 5 April 2018 diff hist +2,054 Purification of His-GST Fusion Protein No edit summary current

4 April 2018

• 17:3517:35, 4 April 2018 diff hist +43,398 Hybridoma production No edit summary current
• 15:3115:31, 4 April 2018 diff hist +1,622 N PCold induction Created page with "The growth/induction conditions (culture medium, culture temperature, aeration, timing of induction, concentration of an inducer, and incubation time after induction) sho..." current

3 April 2018

• 16:1016:10, 3 April 2018 diff hist +410 N Oligo Stock Created page with "1. after receiving oligo, check amount in nmole. 2. add 100 ul TE or EB to 10 nmole oligo (e.g., add 140 ul TE/EB to 14 nmole). Votex and let it sit at RT for 10 min. If it i..." current

2 April 2018

• 22:2922:29, 2 April 2018 diff hist 0 N File:Transfection QC 4 2 2018.JPG No edit summary current
• 12:0212:02, 2 April 2018 diff hist 0 N File:Transfection QC 4 1 2018 small.png No edit summary current

1 April 2018

• 22:4422:44, 1 April 2018 diff hist +3,524 M13 Antibody No edit summary current
• 15:2315:23, 1 April 2018 diff hist +3,594 Virus Like Particles No edit summary current

30 March 2018

• 08:0308:03, 30 March 2018 diff hist +12,675 N FLP recombinase Expression Created page with "Escherichia coli Strain HBlOl  was used for constcuction and propagation of<br/>plasmids. The construction of the substrate plasmids pBAl12, pLB112 and pCS38<br/>has bee..." current

29 March 2018

• 11:0711:07, 29 March 2018 diff hist +1,852 T7 endonuclease Assay No edit summary current

28 March 2018

• 23:0923:09, 28 March 2018 diff hist 0 N File:Protein delivery lipid structure.png No edit summary current
• 23:0323:03, 28 March 2018 diff hist 0 N File:1 2-epoxyohexadecane.png No edit summary current
• 23:0123:01, 28 March 2018 diff hist 0 N File:N- 3-aminopropyl diethaneamine.png No edit summary current
• 22:5622:56, 28 March 2018 diff hist 0 N File:N N-Bis 2-hydroxyethyl ethylene diamine.png No edit summary current
• 22:5122:51, 28 March 2018 diff hist 0 N File:Synthesis of protein delivery lipid.png No edit summary current
• 22:1722:17, 28 March 2018 diff hist +3,639 N Cas9 delivery into cells Created page with "Delivery of proteins complexed with cationic lipids in cell culture Cultured cells were plated in 48-well format (250 µL volume) in Dulbecco’s Modified Eagle’s Media plus..." current

27 March 2018

• 23:4023:40, 27 March 2018 diff hist +6,036 N Enrichment of Antigen-binding B Cells using Magnetic Beads Created page with "Antigen-specific B-cell enrichment and phenotyping BALB/c mice were immunized with 50 μg of pMHCII emulsified in complete Freund's adjuvant (CFA, Sigma) subcutaneously in..." current

23 March 2018

• 11:1511:15, 23 March 2018 diff hist +225 N Amphotericin B Solution for Cell Culture Created page with "Weigh 50 mg Amphotericin B into a sterize tube Add 10 ml ultrapure water to make suspension. Aliquot 1 ml into autoclaved tubes, store at -20 oC Add 0.5 ml to 100 ml 1..." current

22 March 2018

• 20:0320:03, 22 March 2018 diff hist +10,586 Phage Display No edit summary current

21 March 2018

• 10:4910:49, 21 March 2018 diff hist +15,612 N Cloning antibody from B cells Created page with "Immunisations Approval for use of animals for immunisation was provided through the UCB Pharma, UK Animal Welfare and Ethical Review Body (AWERB) and the license was granted b..." current

20 March 2018

• 21:3021:30, 20 March 2018 diff hist +5,026 Peptide design guideline No edit summary current
• 16:1716:17, 20 March 2018 diff hist +1,728 N Strep Tag Created page with "strep-tags I, II and III, nano-tag and streptavidin binding peptide (SBP) tag. These tags bind directly to streptavidin without the need of biotinylation. Although both the na..." current

19 March 2018

• 21:5121:51, 19 March 2018 diff hist +2,385 N Flow Cytometry Antibodies Created page with "Anti-Human CD27 Monoclonal Antibody (O323), Super Bright 436, eBioscience™ (5 tests)<br/> Anti-Human CD86 (B7-2) Monoclonal Antibody (IT2.2), Super Bright 436, eBioscie..." current
• 18:1818:18, 19 March 2018 diff hist 0 N File:3159 EcoR1.JPG No edit summary current