User contributions for Paul.xu

29 May 2019

• 15:1815:18, 29 May 2019 diff hist +775 Muts QC No edit summary current

28 May 2019

• 18:4318:43, 28 May 2019 diff hist 0 File:Muts QC 5-1-19b-gel.jpg Paul.xu uploaded a new version of File:Muts QC 5-1-19b-gel.jpg current
• 16:5516:55, 28 May 2019 diff hist +413 Thermostable MMLV No edit summary current

24 May 2019

• 15:3115:31, 24 May 2019 diff hist +1,628 N Long PCR Created page with "http://arep.med.harvard.edu/labgc/estep/longPCR_protocol.html For PCR with low-complexity templates (e.g., plasmid and cosmid inserts)<br/>50 microliter reaction in 1X Long P..." current

20 May 2019

• 16:5716:57, 20 May 2019 diff hist +450 N Purfication of T4 Polynucleotide Kinase Created page with "== Current Working Protocol == === Protocol 1 === # # # === Protocol 2 === #1 #2 #3 == Based on Literature == === Protocol 1 === Based on [https://www.biotechniques.c..." current
• 15:1715:17, 20 May 2019 diff hist +7,813 Phosphate Buffer Chart No edit summary current

19 May 2019

• 23:3123:31, 19 May 2019 diff hist +662 N Expression Optimization of Cre Recombinase Created page with "5/20/2019, by Paul XU {| border="1" cellspacing="1" cellpadding="1" style="width:650px;" |- | Medium | 5020 | Antiform | Indution | Cell Wet Weight, g | Cre |- | Super Broth,..." current

18 May 2019

• 23:1923:19, 18 May 2019 diff hist 0 N File:Cre purification 5 18 19.jpg No edit summary current

16 May 2019

• 19:4219:42, 16 May 2019 diff hist +245 N Antibody Core and Tools Created page with "University of Maryland Greenebaum Comprehensive Cancer Center Flow Cytometry Shared Service (FCSS) [https://www.medschool.umaryland.edu/cibr/Core/umgccc_flow/ https://www.med..." current

14 May 2019

• 18:2818:28, 14 May 2019 diff hist +211 Super Broth No edit summary current

13 May 2019

• 12:5912:59, 13 May 2019 diff hist +39 N Histag Protein Purification Protocol Created page with "David's Protocol Category:Protocols" current
• 12:5912:59, 13 May 2019 diff hist +198 N Order 5301 Created page with "== Purification of == === Customer === === Method === David's Histag_Purification Protocol === Result ==..." current

12 May 2019

• 16:1216:12, 12 May 2019 diff hist +167 N IT for Biotech Created page with "Hosting [https://www.vultr.com/pricing/ https://www.vultr.com/pricing/] [https://www.digitalocean.com/pricing/ https://www.digitalocean.com/pricing/] Category:IT" current

22 April 2019

• 11:1411:14, 22 April 2019 diff hist +2,107 Transformation No edit summary current

2 April 2019

• 19:5119:51, 2 April 2019 diff hist +545 N Preparation of Products from Stock Created page with "== T3  DNA Ligase == 40 ul stock, + 310 ul storage buffer--> 350 ul, 2.12 mg/ml == T7 DNA Ligase == 270 ul stock + 170 ul storage buffer --> 2.5 mg/ml == 4X Ligase Bu..." current
• 14:1814:18, 2 April 2019 diff hist +1,404 Credit Score History No edit summary current
• 08:2908:29, 2 April 2019 diff hist +34 N Credit History Paul.xu moved page Credit History to Credit Score History current

16 March 2019

• 23:3723:37, 16 March 2019 diff hist +4,814 N Membrane Protein Production Created page with "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2253291/ Spheroplasts of E. coli and a cytoplasmic membrane fraction were prepared according to the protocols by Weiss (1976) and..." current
• 22:5022:50, 16 March 2019 diff hist +117 N Enzymes for feeds Created page with "http://www.bioseutica.com/products http://www.bioseutica.com/products/specialty-products/entegard Category:Ideas" current

14 March 2019

• 11:4511:45, 14 March 2019 diff hist +5,055 Chemicals No edit summary current

8 March 2019

• 11:1011:10, 8 March 2019 diff hist +655 N Aptamer Created page with "TQ21 [https://www.aptagen.com/aptamer/71/taq-dna-polymerase-tq21 https://www.aptagen.com/aptamer/71/taq-dna-polymerase-tq21] Affinity (Kd):36 pM (reported value) Binding Cond..." current

5 March 2019

• 11:2711:27, 5 March 2019 diff hist +1,621 Purification of DNA Binding Protein using Heparin Sepharose No edit summary current
• 10:4810:48, 5 March 2019 diff hist +2,763 Enzymes to be purified No edit summary current

2 March 2019

• 00:1800:18, 2 March 2019 diff hist +82 N 157-2196 QC Created page with "File:3 1 19 Protein QC 2.jpg File:3 1 19 protein QC.jpg Category:QC" current
• 00:1500:15, 2 March 2019 diff hist 0 N File:3 1 19 Protein QC 2.jpg No edit summary current
• 00:1400:14, 2 March 2019 diff hist 0 N File:3 1 19 protein QC.jpg No edit summary current

18 February 2019

• 19:3319:33, 18 February 2019 diff hist +1,180 N Remove DNA from Bacterial Lysate using PEI Created page with "== PEI Precipitation == Dissolve 4 g of PEI in ~ 10 ml of 25 mM Tris-HCl buffer (pH: 8.0). Transfer PEI solution a dialysis tubing (MWCO 3,500 Da) Dialyze against..." current
• 00:4200:42, 18 February 2019 diff hist +14,095 His-Tat-NLS-FLP-Purification No edit summary current
• 00:3900:39, 18 February 2019 diff hist +5,348 Cre expression screening No edit summary current
• 00:0900:09, 18 February 2019 diff hist 0 N File:2115 2108 1708 expression.jpg No edit summary current

7 February 2019

• 19:2519:25, 7 February 2019 diff hist +10,744 Cloning Tasks No edit summary current

21 January 2019

• 12:5412:54, 21 January 2019 diff hist +1,897 High-Yield-Plasmid-Medium No edit summary current

5 January 2019

• 17:3117:31, 5 January 2019 diff hist 0 N File:171 172 digestion1.JPG No edit summary current

2 January 2019

• 14:2414:24, 2 January 2019 diff hist +374 168 pT7-MBP-(tev)-GST-(3c)-sfGFP No edit summary current
• 14:2414:24, 2 January 2019 diff hist +658 165 pT7A3-MBP-(TEV) No edit summary current
• 14:2314:23, 2 January 2019 diff hist 0 N File:165 168 digestion.JPG No edit summary current

1 January 2019

• 16:4016:40, 1 January 2019 diff hist 0 File:2517 166 digestion.JPG Paul.xu uploaded a new version of File:2517 166 digestion.JPG current
• 14:4714:47, 1 January 2019 diff hist +463 167 pT7A3-MBP-TRX-6H No edit summary current

31 December 2018

• 16:1516:15, 31 December 2018 diff hist +47 N 166 pBad24-MBP-(Tev)-GST-(3C)-sfGFP Paul.xu moved page 166 pBad24-MBP-(Tev)-GST-(3C)-sfGFP to 166 pBad-MBP-(Tev)-GST-(3C)-sfGFP current
• 15:4915:49, 31 December 2018 diff hist +49 N 166 pBad24-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H Paul.xu moved page 166 pBad24-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H to 166 pBad24-MBP-(Tev)-GST-(3C)-sfGFP current

27 December 2018

• 10:2810:28, 27 December 2018 diff hist +4,389 Arabinose Induction No edit summary current

26 December 2018

• 16:4916:49, 26 December 2018 diff hist +3,687 Expression of Toxic Protein using pBad Promoter No edit summary current

15 December 2018

• 16:1116:11, 15 December 2018 diff hist +1,786 Precipitation of DNA using Glyogen No edit summary current

8 December 2018

• 15:4815:48, 8 December 2018 diff hist +18,843 Ligation and enzyme-independent assembly methods No edit summary current

7 December 2018

• 18:2318:23, 7 December 2018 diff hist 0 N File:Hot fusion 2.png No edit summary current
• 18:2318:23, 7 December 2018 diff hist 0 N File:Hot fusion 1.png No edit summary current

1 December 2018

• 16:5316:53, 1 December 2018 diff hist +490 PCR Enhancer Mix No edit summary current

30 November 2018

• 18:3618:36, 30 November 2018 diff hist +8,570 N Plasmid Miniprep using CTAB Created page with "== Plasmid DNA CTAB Miniprep from E. coli Preparation == <br/>Label 2 Eppendorf tubes per sample, turn heat block on to 100°C or prepare boiling water bath, prepare fresh ly..." current

29 November 2018

• 20:5520:55, 29 November 2018 diff hist 0 N File:Isopropanol-PEG-Plasmid QC digestion.JPG No edit summary current
• 20:4420:44, 29 November 2018 diff hist 0 N File:PEG ppt Plasmid.JPG No edit summary current