Enzymes for Antibody Engineering
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| Clone ID | Parent ID | Name | Expression or availability | Status | Note |
| pAR1219 |
https://www.sigmaaldrich.com/catalog/product/SIGMA/T2076, $187 |
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| 3248 |
pQE-81L-T7-RNAP-P266L |
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| 1080 | pBad24-RNAPol |
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| 1780 |
1080 |
pT7A3-T7-RNAPol-Y639F |
No | T7 RNA Polymerase Y639F | |
| 1499 |
pT7A30-RNaseInhibitor | Yes |
pig | ||
| 1531 |
pENTR223-RNH1 |
Human RNase Inhibitor, HsCD00513809 | |||
| 1317 |
1316 | pT7A3-RNH1 |
No | extra C, won't translate | |
| 1316 | pLX304-RNH1 |
human ribonuclease/angiogenin inhibitor | |||
| 1536 |
1531 |
pT7A3-RNH1 |
No |
Human RNase Inhibitor. Need to co-express with GroELS, induction at 22 oC and DTT in, 0.04% arabinose, see https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-65 | |
| 1495 |
501 |
pT7A3-MMLV | No | ||
| 501 | pET30b-MMLV |
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| 1713 | MMLV |
wt MMLV from Mingjie | |||
| 1714 |
1549 |
pEC-iMMLV |
superscript (E69K,E302R,N454K,D524N) | ||
| 1738 |
1714 |
pEC-iMMLV-m |
E69K,E302R,W313F,L435G,N454K,D524N | ||
| 1549 |
1713 |
pEC-MMLV-wt |
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| 1858 |
1713 |
pEC-fMMLV |
D200N, L603W, T330P, L139P and E607K | ||
| 1747 | 1738 | pT7A30-iMMLV |
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| 1868 |
1858 |
pT7A30-fMMLV |
Y, 2 | D200N, L603W, T330P, L139P and E607K, thermostable at 62°C, has expression | |
| 1706 |
pT7A3-BirA | Y | Biotin ligase | ||
| 1498 |
pT7A3-TaqDNALigase | ||||
| 1035 |
pET28b-Tth-Ligase-K294R |
high fiderity | |||
| 1735 | pT7A30-Exo-Taq-sso7d | Taq-sso7D | |||
| KOD | |||||
| Phusion | |||||
| 1765 |
1619 | pT7A3-T4DNAPol | Yes | T4 DNA Polymerase | |
| 1619 |
pET28b-T4DNAPol |
T4 DNA Polymerase | |||
| T4 DNA ligase | |||||