User contributions for Paul.xu

Results for Paul.xu talk block log uploads logs

A user with 13,447 edits. Account created on 25 October 2010.

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16:0516:05, 26 March 2012 diff hist +1,221 1021-pET8XHis-T5Exo No edit summary current
16:0316:03, 26 March 2012 diff hist 0 N File:1011+1017+1021 digest detect 3-26-2012.JPG No edit summary current

12:1712:17, 25 March 2012 diff hist +1,092 1020-TurboRFP-TA vector No edit summary current
12:1612:16, 25 March 2012 diff hist 0 N File:1020-TurboRFP-TA vector Xho1 Xba1.jpg No edit summary current
12:1612:16, 25 March 2012 diff hist 0 N File:1020-TurboRFP-TA vector EcoR1.jpg No edit summary current
11:3811:38, 25 March 2012 diff hist +811 1037 pET30b-T7ligase No edit summary current
11:3611:36, 25 March 2012 diff hist 0 File:T7 ligase digestion 2012 3 25.jpg uploaded a new version of "File:T7 ligase digestion 2012 3 25.jpg" current
11:3011:30, 25 March 2012 diff hist 0 N File:T7 ligase 2nd PCR2 2012 3 25.jpg No edit summary current
11:3011:30, 25 March 2012 diff hist 0 N File:T7 ligase 2nd PCR 2012 3 25.jpg No edit summary current
11:2911:29, 25 March 2012 diff hist 0 N File:T7 ligase 1st PCR 2012 3 25.jpg No edit summary current

22:4722:47, 24 March 2012 diff hist +29,758 NEB DNA Polymerase No edit summary current

17:4717:47, 22 March 2012 diff hist +7,515 Tn5 Transposase No edit summary current
14:1314:13, 22 March 2012 diff hist 0 N File:1025clone 3-22-2012.JPG No edit summary current
14:1314:13, 22 March 2012 diff hist 0 N File:1011+1017+1021clone 3-22-2012.JPG No edit summary current
14:1314:13, 22 March 2012 diff hist 0 N File:TurboRFP-TA digest detect 3-22-2012.JPG No edit summary current

15:5115:51, 21 March 2012 diff hist +4,667 NEB IMPACT No edit summary current
15:2215:22, 21 March 2012 diff hist 0 N File:1021 PCR 3-21-2012.JPG No edit summary current
15:2215:22, 21 March 2012 diff hist 0 N File:1011+1017 PCR 3-21-2012.JPG No edit summary current

10:4610:46, 19 March 2012 diff hist +2,102 523-pCDNA3.1-Bcl-2 No edit summary current
10:4510:45, 19 March 2012 diff hist 0 N File:523 digest detect 3-19-2012.JPG No edit summary current

14:2514:25, 18 March 2012 diff hist +2,328 N Error Prone PCR Created page with "=== <br>Materials === 1. Appropriate PCR amplification primers, designed to have similar melting temperatures, stored at –20°C (see Note 2).<br>2. Plasmid containing gene of ..." current

16:0516:05, 17 March 2012 diff hist +1,973 N RedB Purification Created page with "== Protocol 1 == 1. Grow pBD24-RedB in BL21-AI (AI: Arabinose inducible) at 37 °C.<br>2. Dilute overnight culture 1/50 into 1 liter of LB-Amp, grown to an OD600 value of 0.5.<b..." current
00:1000:10, 17 March 2012 diff hist +2,261 N Confusion Cloning Created page with "Confusion PCR Cloning == Home made extract == <br>1, Grow DH10B or Top10 with pBad24-Red-alpha at 37 oC in 100 ml 2X YT medium until they reached OD600 ~ 5.3.<br>2. Add 0.2% L-..." current

23:3523:35, 16 March 2012 diff hist +12,679 SLIC, Gibson and CPEC assembly No edit summary current
22:4622:46, 16 March 2012 diff hist 0 N File:J5 6.png No edit summary current
22:4622:46, 16 March 2012 diff hist 0 N File:J5 5.png No edit summary current
22:4522:45, 16 March 2012 diff hist 0 N File:J5 4.png No edit summary current
22:4522:45, 16 March 2012 diff hist 0 N File:J5 3.png No edit summary current
22:4522:45, 16 March 2012 diff hist 0 N File:J5 2.png No edit summary current
22:4522:45, 16 March 2012 diff hist 0 N File:J5 1.png No edit summary current
17:5717:57, 16 March 2012 diff hist +10,800 Taq377 Purification No edit summary current
17:0317:03, 16 March 2012 diff hist +1,068 N Real-time PCR buffer Created page with "The PCR buffer consisted of 20 mM Tris-HCl, pH 8.4, 50 mM KCl, 4.5 mM MgCl2, 0.05% Nonidet™ P-40, 0.05% Tween® 20, 0.03 % acetylated bovine serum albumin (BSA), and 200 μM ea..." current
15:5215:52, 16 March 2012 diff hist 0 N File:523 digest detect 3-16-2012.JPG No edit summary current
11:1611:16, 16 March 2012 diff hist +4,885 Isothermal Assembly No edit summary current

15:1615:16, 15 March 2012 diff hist 0 N File:1009 digest detect 3-15-2012.JPG No edit summary current
10:2910:29, 15 March 2012 diff hist +2,419 HTNCre Purification Mep No edit summary current
10:2810:28, 15 March 2012 diff hist 0 N File:Cre 2012 3 8.jpg No edit summary current
10:1110:11, 15 March 2012 diff hist 0 N File:Cre purification 3 15 2012 2.jpg No edit summary current
10:1110:11, 15 March 2012 diff hist 0 N File:Cre purification 3 15 2012.jpg No edit summary current

13:1113:11, 14 March 2012 diff hist 0 N File:523 clone 3-14-2012.JPG No edit summary current
13:1113:11, 14 March 2012 diff hist 0 N File:523pPCR 3-13-2012.JPG No edit summary current
13:0313:03, 14 March 2012 diff hist 0 N File:523pPCR 3-12-2012.JPG No edit summary current

23:3523:35, 13 March 2012 diff hist +378 Gene Synthesis No edit summary current

11:1611:16, 12 March 2012 diff hist 0 N File:Cre MEP purification march 2012.jpg No edit summary current
10:2610:26, 12 March 2012 diff hist 0 N File:Cre purification mep march 2012.jpg No edit summary current

23:3323:33, 11 March 2012 diff hist +3,269 N Lentivirus RT-PCR Created page with "1. Seed 293T cells were seeded in a two-tray Nunc A/S cell factory (CF2) at 60x10^6 cells per cell factory in OPTIMEM with glutamax supplemented with 2% FCS and 20 μg/ml gentami..." current
23:0523:05, 11 March 2012 diff hist +241 N Lentivirus Concentration poly-L-lysine Created page with "1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS) 2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4..." current

10:4810:48, 9 March 2012 diff hist +3,937 Synthesis PHP-sys-DSP-gPEI No edit summary current
10:4510:45, 9 March 2012 diff hist +2,150 Transfection reagents 2012-1-4 No edit summary current

16:0816:08, 7 March 2012 diff hist 0 N File:Turbo-RFP digest detect 3-7-2012.JPG No edit summary current

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