User contributions for Paul.xu

22:2422:24, 24 June 2017 diff hist 0 Category:To-do Blanked the page current
21:5121:51, 24 June 2017 diff hist +2,352 N PEG Precipiate PCR Product Created page with "'''PEG Precipitation of PCR products''' 1, Run 50 ul PCR 2, Add 50 µL of PEG to a 0.5 mL tube. Transfer the remainder of the PCR to the tube with PEG and mix by pipetting u..." current
21:0021:00, 24 June 2017 diff hist +4,101 N PEG Maxiprep Created page with "= Large scale DNA Purification by PEG precipitation = == Perparation of Crude Lysate by Alkaline Lysis == 1. Inoculate 500mL LB media with and grow O/N at 37oC until cu..." current
20:5220:52, 24 June 2017 diff hist +657 N Precipitate DNA with PEG Created page with "2X Precipitation Solution: 40% (W/V) PEG 8000, 20 mM NaCl2 To DNA solution add PEG concentration of 13% in the presence of 10 mM MgCl2 at room tempera..." current
13:2413:24, 24 June 2017 diff hist 0 N File:832 837 EcoRV digestion.JPG No edit summary current
11:2111:21, 24 June 2017 diff hist +1,396 MiniPrep using Spin Column No edit summary current

17:5217:52, 23 June 2017 diff hist 0 File:837 pMiniT-Hia5 PCR Screening.JPG Paul.xu uploaded a new version of File:837 pMiniT-Hia5 PCR Screening.JPG current
17:5017:50, 23 June 2017 diff hist 0 File:832 pMiniT-VVTopo PCR Screening.JPG Paul.xu uploaded a new version of File:832 pMiniT-VVTopo PCR Screening.JPG current
12:0112:01, 23 June 2017 diff hist +2,855 N PCR Screening and Restriction Digestion Created page with "== PCR Screening == === Screening of clonies === 1.Aliquot 5 ul of autoclaved H2O to 500 ul tubes. 2. Pick each individual colony, suspend in 5 ul H2O 3. Transfer 0...." current
11:5411:54, 23 June 2017 diff hist +1,001 PCR from Plasmid and Genomic DNA No edit summary current

12:2212:22, 22 June 2017 diff hist +672 N Ligation Enhancers Created page with "US20120283144 12%1,2-propanediol 14-fold 5-10% PEG 6000 Ethylene glycol (12%) 10-fold In addition to 5-10% PEG 6000 5%-15% glycerol and 1-8% 1,2-PrD Were &nbs..." current
11:5511:55, 22 June 2017 diff hist +1,455 N Star Activity Created page with "the rate difference for EcoRI at its cognate site (5´-GAATTC-3´) and next best site (5´-TAATTC-3´) is of the order of 105 (1) . Similarly, for EcoRV, cleavage at its cogna..." current
08:1108:11, 22 June 2017 diff hist +4,343 Restriction Methylase No edit summary current

23:3223:32, 21 June 2017 diff hist +2,123 Enzyme stabilizer No edit summary current
22:5222:52, 21 June 2017 diff hist +1,098 N NID Miniprep Created page with "NID miniprep plasmid isolation procedure Spin down 2 ml~5 ml of bacterial cultures. Add 200 µl E-Buffer. Resuspend cells by votexing Incubate cell suspension at 65..." current
16:1216:12, 21 June 2017 diff hist +1,402 Sequencing No edit summary current
16:0716:07, 21 June 2017 diff hist +4,222 Blunt-end PCR Cloning No edit summary current
13:2013:20, 21 June 2017 diff hist +207 N Antibody Services Created page with "[https://www.uth.edu/imm/service-centers/antibody-engineering-and-expression-service-center/ https://www.uth.edu/imm/service-centers/antibody-engineering-and-expression-servic..." current
10:3110:31, 21 June 2017 diff hist +165 CutSmart Buffer No edit summary current

13:1513:15, 16 June 2017 diff hist +8,298 N Purification of T4 DNA Ligase Created page with "== Protocol 1 == Preparation of Cell Extract Unless otherwise noted, all steps were performed at 4°C. One hundred grams of frozen cells were thawed and resuspended in 400 m..." current

18:2418:24, 12 June 2017 diff hist +69 N Gene Synthesis Rare Codon Analysis Created page with "http://people.mbi.ucla.edu/sumchan/caltor.html Category:Protocols" current
16:0416:04, 12 June 2017 diff hist +475 N Restriction Digestion Created page with "'''Digestion''' Use 5 ul miniprep for digestion 1. make mix, per reaction 1.5 ul FastDigestion Buffer 0.3 ul enzyme 1 0.3 ul enzyme 2 (if required) 5 ul miniprep..." current
15:4615:46, 12 June 2017 diff hist +334 N Make Miniprep From Plate Created page with "1. Transform plasmid stock with 100 ul of NEB Turbo. 2. Grow on LB agar plate with antibiotics o/n at 37 oC. 3. Add 1.5 ml ddH2O to plate to suspend bacteria from agar..." current

10:1910:19, 9 June 2017 diff hist +1,095 N Toxic Minigene Created page with "5'-C UGAACGAGAAACGU AAA '''AUGAUAUAA'''AUAUCAA. 3' barl $ ' -CAUC'''AGGA A'''UAUUUGAUUCAG '''AUG AUA UGA '''CUAUCAA- 3' bar ll Me..." current

19:0519:05, 7 June 2017 diff hist +1,398 N Gene Synthesis by Annealing Created page with "Design oligos using: Gene2Oligo software ([http://berry.engin.umich.edu/gene2oligo http://berry.engin.umich.edu/gene2oligo]) Mix 1 μl of each oligonucleotide (from 100 ..." current
18:5418:54, 7 June 2017 diff hist +952 N Cre Activity Assay Created page with "Grow 293T in 12 well plate. Transformation of 293T cells with 1 ug 2181..." current

12:2712:27, 30 May 2017 diff hist +3,386 N Bioreducible Zinc Coordinative PEI Created page with "For DDAC synthesis, 2,2’-dipicolylamine (DPA), alpha,alpha'-dichloro-p-xylene (Dx), 5-amino-1-pentanol (AP), chlorpromazine (CPZ) and amiloride hydrochloride (AM) were purch..." current

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